A single betaproteobacterium dominates the microbial community of the crambescidine-containing sponge Crambe crambe
نویسندگان
چکیده
Crambe crambe is a marine sponge that produces high concentrations of the pharmacologically significant pentacyclic guanidine alkaloids (PGAs), Crambescines and Crambescidines. Although bio-mimetic chemical synthesis of PGAs suggests involvement of microorganisms in their biosynthesis, there are conflicting reports on whether bacteria are associated with this sponge or not. Using 16S rRNA gene pyrosequencing we show that the associated bacterial community of C. crambe is dominated by a single bacterial species affiliated to the Betaproteobacteria. Microscopy analysis of sponge tissue sections using a specific probe and in situ hybridization confirmed its dominance in the sponge mesohyl and a single microbial morphology was observed by transmission electron microscopy. If confirmed the presence of a simple bacteria community in C. crambe makes this association a very pertinent model to study sponge-bacteria interactions and should allow further research into the possible implication of bacteria in PGA biosynthesis.
منابع مشابه
Culture-dependent and independent approaches for identifying novel halogenases encoded by Crambe crambe (marine sponge) microbiota
Sponges harbour microbial communities that contribute to the genetic and metabolic potential of their host. Among metabolites produced by sponge-associated microbial communities, halogenated compounds are of special interest because of their biotechnological potential. In this study, we have examined the diversity of the cultivable fraction of the marine demosponge Crambe crambe microbiota. App...
متن کاملPhylogeographical history of the sponge Crambe crambe (Porifera, Poecilosclerida): range expansion and recent invasion of the Macaronesian islands from the Mediterranean Sea.
We studied sequence variation in the nuclear ribosomal internal transcribed spacers (ITS-1 and ITS-2) in 111 individuals from 11 populations/localities of the sponge Crambe crambe across the core species range in the western Mediterranean Sea and Atlantic Ocean. We report the first confirmed instance of intragenomic variation in sponges. Phylogeographical, nested clade and population genetic an...
متن کاملPhylogeography, gene Flow and Population Structure of Crambe crambe (Porifera: Poecilosclerida)
متن کامل
Spherulization as a process for the exudation of chemical cues by the encrusting sponge C. crambe
Ecological interactions in the marine environment are now recognized to be partly held by chemical cues produced by marine organisms. In particular, sponges are sessile animals thought to rely on the bioactive substances they synthesize to ensure their development and defense. However, the mechanisms leading the sponges to use their specialized metabolites as chemical cues remain unknown. Here ...
متن کاملInfluence of crambe meal as a protein source on intake, site of digestion, ruminal fermentation, and microbial efficiency in beef steers fed grass hay.
Four ruminally and duodenally cannulated beef steers (558 +/- 37 kg) were arranged in a 4 x 4 Latin square to evaluate the influence of crambe meal as a protein source on intake, digestion, and microbial efficiency. Steers were offered chopped (10.2 cm) brome hay (6.2% CP) for ad libitum consumption and one of four supplements. Protein sources used were soybean and crambe meals (CM). Protein so...
متن کامل